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Principal Scientist Streptomyces Molecular Biology/Genetics

Pfizer


Location:
Kalamazoo, MI
Date:
12/06/2017
2017-12-062018-01-06
Pfizer
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Job Details

**ROLE SUMMARY**



The position of Principle Scientist - Streptomyces Molecular Biology/Genetics will provide scientific leadership as well as working as part of an interdisciplinary team to improve Pfizer's production processes through genetic manipulation of Streptomyces to enhance production of secondary metabolites, biocatalysis and microbial culture improvement projects.







Principal duties include genetic manipulation of Streptomyces to enhance production of secondary metabolites, pathway, metabolic and enzyme engineering, heterologous gene expression, enzyme assays, knowledge of methods for analysis of CoA-ester pools which are the building blocks for many antibiotics, high through-put screening and development of new molecular biology tools to accomplish these tasks.







**ROLE RESPONSIBILITIES**







+ Build robust production strains, and work with fermentation scientists to identify desirable culture traits and to develop the production process for important industrial microorganisms including yeast, bacterial and fungal strains.



+ Improve production strains by molecular biological methods and by traditional mutagenesis and screening.



+ Determine and understand metabolic or reaction pathways and genetically engineer those pathways for improved culture performance and/or to produce new APIs.



+ Adapt, develop and implement molecular genetics tools for manipulating industrial organisms including protein expression, cloning and DNA assembly, gene replacement, novel and rapid gene knockout technologies (transposon, PCR-based, CRISPR)



+ Remain current with and champion the implementation of new technologies within the group to ensure we deliver or exceed project targets.







**QUALIFICATIONS**







+ 10+ Years with BS



+ 4+ Years with PhD in related field







Experience:







+ Experience working with genetic modification of E. coli , actinomycetes, yeasts, and/or other important industrial micro-organisms.







+ Demonstrated technical expertise to amplify and clone large gene clusters of Streptomyces using Gibson Assembly







Technical Skills







+ Expertise in strain improvement by molecular biological methods and traditional mutagenesis/screening.



+ Expertise in genetic, metabolic and pathway engineering for improving existing products and creating new biosynthetic pathway for novel metabolites.



+ Hands-on technical experience and demonstrated success in amplification of complete biosynthetic clusters in Streptomyces using Gibson Assembly



+ Experience in working with and characterizing Fungal Cytochrome P450s



+ Experience with protein evolution programs and methods



+ Strong knowledge of molecular genetics techniques, business and bioinformatic software, and operation of molecular biology related instrumentation especially with industrially relevant microorganisms including actinomycetes.



+ Ability to design experiments as well as to analyze, interpret, follow-up and communicate experimental data.



+ Strong initiative and good problem solving skills. Takes personal responsibility for own work, pushing self and others to exceed goals and deliver results.



+ Must have demonstrated communication skills, both written and oral.



+ Must be able to establish positive colleague relationships which foster collaboration and cooperation. BDG is a team-based organization. Good peer relationships are the only way to get projects done and to succeed.



+ Change agile - can effectively cope with change.







**PHYSICAL/MENTAL REQUIREMENTS**







+ Lab position: Frequent standing and walking, frequent detailed manual manipulations, and computer-based work.



**NON-STANDARD WORK SCHEDULE, TRAVEL OR ENVIRONMENT REQUIREMENTS**







+ First shift position - some overtime and/or weekend work may be required







**OTHER INFORMATION**







+ Internal candidate identified



+ Not eligible for relocation







**EEO & Employment Eligibility**



Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.







**Sunshine Act**



Pfizer reports payments and other transfers of value to health care providers as required by federal and state transparency laws and implementing regulations. These laws and regulations require Pfizer to provide government agencies with information such as a health care provider's name, address and the type of payments or other value received, generally for public disclosure. Subject to further legal review and statutory or regulatory clarification, which Pfizer intends to pursue, reimbursement of recruiting expenses for licensed physicians may constitute a reportable transfer of value under the federal transparency law commonly known as the Sunshine Act. Therefore, if you are a licensed physician who incurs recruiting expenses as a result of interviewing with Pfizer that we pay or reimburse, your name, address and the amount of payments made currently will be reported to the government. If you have questions regarding this matter, please do not hesitate to contact your Talent Acquisition representative.







+ Last Date to Apply for Job: December 19, 2017.







Pfizer is an equal opportunity employer and complies with all applicable equal employment opportunity legislation in each jurisdiction in which it operates.
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