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Senior Scientist, Protein Expression and Analytics


Cambridge, MA
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Job Details

Job Title Senior Scientist, Protein Expression and Analytics

JobID 1604830

Location: Cambridge, MA

Description Position purpose :

The Target Protein Engineering group within the Biomedicine Design department seeks a protein expression and analytics scientist tasked with assisting in the expression and characterization of target proteins and antibodies in transient and stable eukaryotic expression systems to support immune protein screening, activity assays, binding assays, structural characterization, and/or to advance knowledge of candidate therapeutic proteins.

Primary responsibilities :

+ Transient expression of soluble and cell surface proteins in eukaryotic expression systems, including mammalian cells, insect cells, and yeast.

+ Development and use of assays to support the analysis of recombinant proteins and cell lines using flow cytometry, ELISA, Octet, and other common analytic techniques.

+ Creation of stable mammalian and insect cell lines expressing recombinant target proteins or antibodies

Technical skill requirements :

+ Strong proficiency in flow cytometry (including cell sorting) and associated software tools (e.g. FLowJo) is essential.

+ Demonstrated competency in development and optimization of ELISA and related immunoassays is highly desirable.

+ Experience with mammalian cell culture and stable cell line generation is strongly preferred.

+ Familiarity with E.coli expression systems is desirable but not essential.

Qualifications :

+ Ph.D in life sciences with 2+ years or BS/MS sciences with 6+ years hands-on bench experience in a Pharmaceutical or Biotech setting working on scientific projects directly relevant to requirements for this position

+ Strong collaborative and interpersonal skills, and ability to work effectively in a team environment

+ Excellent oral and written communication skills

+ High level of precision, accuracy, and attention to detail is essential.

+ Ability to manage multiple, sometimes disparate projects in parallel, while maintaining attention to detail

A career at Pfizer offers opportunity, ownership and impact.

All over the world, Pfizer colleagues work together to positively impact health for everyone, everywhere. Our colleagues have the opportunity to grow and develop a career that offers both individual and company success; be part of an ownership culture that values diversity and where all colleagues are energized and engaged; and the ability to impact the health and lives of millions of people. Pfizer, a global leader in the bio-pharmaceutical industry, is continuously seeking top talent who are inspired by our purpose to innovate to bring therapies to patients that significantly improve their lives.

Pfizer is an equal opportunity employer and complies with all applicable equal employment opportunity legislation in each jurisdiction in which it operates.

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