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Sr. Associate Scientist, Protein Expression, Cell Biology


Cambridge, MA 02139
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Job Details

The Target Protein Engineering group within the Pfizer Biomedicine Design department seeks a protein expression scientist tasked with assisting in the expression of target proteins and antibodies in transient and stable eukaryotic expression systems to support immune protein screening, activity assays, binding assays, structural characterization, and/or to advance knowledge of candidate therapeutic proteins.

Primary responsibilities :

+ Creation and characterization of stable mammalian cell lines for the production of target proteins for antibody discovery and validation.

+ Culturing and maintenance of mammalian cell lines

+ Characterization and documentation of expression results using gel electrophoresis, immunoblotting, flow cytometry, and other techniques

+ Working as a team with group members to plan and prioritize expression queue and scale, to provide conditioned media or extracts to protein biochemists in expected time frame.

Qualifications :

+ Minimum of BS degree in biological sciences, with 1+ years direct relevant hands-on experience in a pharmaceutical, biotech, or academic laboratory

+ Proficiency in growth and maintenance of eukaryotic cell cultures is required.

+ Demonstrated competency with various protein characterization methods (e.g. SDS-PAGE, Western blotting, ELISA).

+ Experience with small-scale protein purification is desirable.

+ Proficiency with optimizing and troubleshooting instrumentation is desirable

**EEO & Employment Eligibility**

Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.

**Sunshine Act**

Pfizer reports payments and other transfers of value to health care providers as required by federal and state transparency laws and implementing regulations. These laws and regulations require Pfizer to provide government agencies with information such as a health care provider's name, address and the type of payments or other value received, generally for public disclosure. Subject to further legal review and statutory or regulatory clarification, which Pfizer intends to pursue, reimbursement of recruiting expenses for licensed physicians may constitute a reportable transfer of value under the federal transparency law commonly known as the Sunshine Act. Therefore, if you are a licensed physician who incurs recruiting expenses as a result of interviewing with Pfizer that we pay or reimburse, your name, address and the amount of payments made currently will be reported to the government. If you have questions regarding this matter, please do not hesitate to contact your Talent Acquisition representative.

**Other Job Details:**

+ **Last Date to Apply for Job:** March 1, 2018

+ Eligible for Employee Referral Bonus: yes

Pfizer is an equal opportunity employer and complies with all applicable equal employment opportunity legislation in each jurisdiction in which it operates.
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